The potential of intracellular 13C MR spectroscopy to study the absolute configuration of endogenous and polarized alanine

Natalia Leksa
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Anna Pękala
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Piotr Pardak
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Rafał Dziedzic
Dorota Bartusik-Aebisher
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Rafal Filip
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Sabina Galiniak
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David Aebisher
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Institute of Medical Sciences, Medical College, University of Rzeszow, Rzeszow, Poland
Department of Gastroenterology with IBD Unit, Clinical Hospital No. 2, Rzeszów, Poland
Department of Gastroenterology with IBD Unit, Clinical Hospital No. 2, Rzeszów, Poland
Department of Neurosurgey, Hospital MSWiA, Rzeszow, Poland
Department of Biochemistry and General Chemistry, Medical College of the University of Rzeszow, University of Rzeszow, Rzeszow, Poland
Department of Gastroenterology, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland
Department of Biochemistry and General Chemistry Medical College of Rzeszów University, Rzeszów, Poland
Department of Photomedicine and Physical Chemistry, Medical College of Rzeszów University, Rzeszów, Poland
Received: 6 November 2019 / Accepted: 13 January 2020 / Published: 30 March 2020

Abstract

Introduction. Quantitative and accurate monitoring of tumor makes hyperpolarized carbon (13C) Magnetic Resonance Imaging and Spectroscopy (MRI/S) a powerful tool for in vivo metabolic and structural study. Moreover, the studies of the properties and functions in tumor tissue of the compounds of carbon (C) that are organic, are fundamental to tumor biochemistry.

Aim. To review 13C MR spectroscopy to study the absolute configuration of endogenous and polarized alanine Material and methods. An analysis of literature regarding 13C MR spectroscopy of polarized alanine.

Results. Current evidence suggests that the determination of absolute configurations of amino acids play significant role in physiological mechanisms during tumor growth and treatment.

Conclusions. Nearly 50% nuclear polarization for 13C can be achieved in various organic molecules when Dynamic Nuclear Polarization DNP is performed in a strong magnetic field and at cryogenic temperatures. 

 

Cite

Leksa N, Pękala A, Pardak P, et al. The potential of intracellular 13C MR spectroscopy to study the absolute configuration of endogenous and polarized alanine. Eur J Clin Exp Med. 2020;18(1):20–23. doi: 10.15584/ejcem.2020.1.4

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