Zn and Se supplementation abrogated metals-(metaloids) mixture mediated ocular-thymus toxicity via modulation of oxido-inflammatory and antiapoptotic mechanisms in female Sprague Dawley rats

Mfoniso Antia
Anthonet N. Ezejiofor
Chinna N. Orish
Theresa Ugwu
Ana Cirovic
Aleksandar Cirovic
Doris N. Ajibo
Orish E. Orisakwe
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African Centre of Excellence for Public Health and Toxicological Research (ACE-PUTOR), University of Port Harcourt, Choba, Nigeria
African Centre of Excellence for Public Health and Toxicological Research (ACE-PUTOR), University of Port Harcourt, Choba, Nigeria
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Choba, Nigeria
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University, Elele, Rivers State, Nigeria
University of Belgrade, Faculty of Medicine, The Institute of Anatomy, Belgrade, Serbia
University of Belgrade, Faculty of Medicine, The Institute of Anatomy, Belgrade, Serbia
Department of Experimental Pharmacology and Toxicology, Faculty of Pharmacy, University of Port Harcourt, Choba, Nigeria
African Centre of Excellence for Public Health and Toxicological Research (ACE-PUTOR), University of Port Harcourt, Choba, Nigeria
Received: 25 May 2024 / Revised: 17 June 2024 / Accepted: 29 July 2024 / Published: 30 December 2024

Abstract

Introduction and aim. This is an evaluation of the protective effects of Zn and Se in the eye and thymus of rats exposed to cock tail noxious metal mixtures (CNMM) (Al, Pb, Hg and Mn) in ameliorating ocular pathologies due to autoimmunity.

Material and methods. Female Sprague rats were grouped into eight (n=5) and orally exposed to various treatments for a period of 60 days: (1): the control group receive deionized water only; (2): the CNMM only group received lead acetate Pb(C2H3O2)2 (20 mg/kg), AlCl3 (35 mg/kg), HgCl2 (0.40 mg/kg) and MnCl2 (0.56 mg/kg); (3) received CNMM+ZnCl2, 0.80 mg/kg; (4) received CNMM+Na2SeO3, 1.50 mg/kg; (5) received CNMM+ZnCl2, 0.80 mg/kg and Na2SeO3, 1.50 mg/kg combination. Oxidative stress markers, nuclear factor erythroid 2-related factor 2, nuclear factor kappa B, interleukin 6, tumor necrosis factor alpha and caspase-3 and histopathological changes were determined.

Results. CNMM decreased antioxidants levels but increased malondialdehyde and nitric oxide concentrations. CNMM in creased levels of nuclear factor erythroid 2- related factor 2, and nuclear factor kappa B, interleukin 6 and tumor necrosis factor alpha and caspase-3. There was moderate retinal degeneration and total cell loss at the ganglionic cell layer in the eye; severe degenerative thymus, lymphocyte depletion and multifocal necrosis in CNMM only.

Conclusion. Supplementation with Zn and Se reduced the biochemical and histopathological changes in the eye and thymus in response to CNMM exposure.

 

Cite

Antia M, Ezejiofor AN, Orish CN, et al. Zn and Se supplementation abrogated metals-(metaloids) mixture-mediated ocular-thymus toxicity via modulation of oxido-inflammatory and antiapoptotic mechanisms in female Sprague Dawley rats. Eur J Clin Exp Med. 2024;22(4):870–884. doi: 10.15584/ejcem.2024.4.30.

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