Phytochemical analysis, stability, and antimicrobial activity of eighteen medicinal plants studied against five multi-drug resistant human pathogens

G. Dharshana Malya
Jhumpa Bhattacharjee
Sahana Mukherjee
Shubhajyoti Deka
Lahari Saikia
Shoma Paul Nandi
ID
Email src
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
Assam Medical College and Hospital, Dibrugarh, Assam, India
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
Assam Medical College and Hospital, Dibrugarh, Assam, India
Assam Medical College and Hospital, Dibrugarh, Assam, India
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
Received: 22 March 2024 / Revised: 12 June 2024 / Accepted: 24 June 2024 / Published: 30 December 2024

Abstract

Introduction and aim. Several medicinal plants from India have been reported to be effective against human pathogens, but comprehensive comparative studies are scarce. The aim of this study has been to evaluate and compare the antimicrobial activity, phytochemical composition, and stability at different temperatures and pH of ethanolic extracts of eighteen Indian medicinal plants which are prevalent in North-east India and thrive abundantly.

Material and methods. These plants included Syzygium cumini, Cannabis sativa, Camellia sinensis, Murraya koenigii, Alstonia scholaris, Terminalia chebula, Flemingia strobilifera, Azadirachta indica, Prunus persica, Euphorbia thymifolia, Averrhoa carambola, Paderia foetida, Psidium guajava, Spondias pinnata, Garcinia cowa, Litsea cubeba, Micania macrantha, and Phlogocanthus thyrsiflorus. Their potent medicinal properties made them the ideal choice for this study which included the use of agar well diffusion method and phytochemical analysis.

Results. Agar well diffusion has been used to test their antibacterial activity, which demonstrated higher antibacterial activity of G. cowa extract against Vibrio cholerae and Staphylococcus aureus, whereas S. pinnata extract was most effective against V. cholerae and Enterococcus faecium.

Conclusion. Phytochemical analysis revealed the presence of tannins, alkaloids, saponins, glycosides, steroids, terpenoids, flavonoids, and phenols. The antimicrobial activity of these plant extracts remained stable at higher temperatures and varying pH levels.

 

Cite

Malya GD, Bhattacharjee J, Mukherjee S, Deka S, Saikia L, Nandi SP. Phytochemical analysis, stability, and antimicrobial activity of eigh teen medicinal plants studied against five multi-drug resistant human pathogens. Eur J Clin Exp Med. 2024;22(4):811–819. doi: 10.15584/ ejcem.2024.4.17.

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited