The effect of thiocetam on the parameters of the nitric oxide system under the conditions of the experimental periodontitis and immobilization stress formation

Mykhailo Regeda
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Petro Olekshij
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Maaryana Regeda-Furdychko
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Lubomur Furdychko
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Marta Kolishetska
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Stepan Regeda
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Vitalii Fil
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Department of Pathophysiology Danylo Halytsky, Lviv National Medical University, Lviv, Ukraine
Department of Orthopedic Dentistry, Private Higher Education Institution “Lviv Medical University”, Lviv, Ukraine
Department of Anatomy, Physiology, Pathology, Private Higher Education Institution “Lviv Medical University”, Lviv, Ukraine
Department of Anatomy, Physiology, Pathology, Private Higher Education Institution “Lviv Medical University”, Lviv, Ukraine
Department of Pathophysiology Danylo Halytsky, Lviv National Medical University, Lviv, Ukraine
Department of Surgical and Orthopedic Stomatology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Department of Medical and Biological Disciplines, Geography, and Ecology, Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
Received: 25 October 2023 / Revised: 14 January 2024 / Accepted: 2 February 2024 / Published: 30 June 2024

Abstract

Introduction and aim. The aim of this work is to study the parameters of the nitric oxide (NO) system in the blood of guinea pigs under the conditions of the experimental periodontitis (EP) and immobilization stress (IS) formation and to evaluate the effectiveness of thiocetam use.

Material and methods. Experimental studies were performed on 50 guinea pigs (males, body weight 0.18–0.21 kg) which were divided into five groups (10 in each): the first group were intact animals as control; the second experimental group were animals with experimental periodontitis under conditions of immobilization stress (3rd day), the third group included guinea pigs with EP and IS on the 5th day of the combined model process, group IV – animals with EP and IS 15th day (without administration of thiocetam) and group V – animals on the 15th day of experiment with EP and IS after use of thiocetam.

Results. As a result of this research, changes in the activity of the NO system in the blood were observed, namely an increase in the level of stable metabolites and an increase in the activity of total NO-synthase, which is accompanied by a compensatory inhibition of the L-arginine activity, and these indicators were most pronounced in the late stages of EP and IS formation.

Conclusion. The use of thiocetam showed a corrective effect on the changed variables of NO metabolism in the peripheral blood of guinea pigs under the conditions of the EP and IS development.

 

Cite

Regeda M, Olekshij P, Regeda-Furdychko M, Furdychko L, Kolishetska M, Regeda S, Fil V. The effect of thiocetam on the parameters of the nitric oxide system under the conditions of the experimental periodontitis and immobilization stress formation. Eur J Clin Exp Med. 2024;22(2):340–346. doi: 10.15584/ejcem.2024.2.16.

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